Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model

Research performed at:

1.Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, University of Cordoba, Córdoba, Spain.

2. Faculty of Veterinary Sciences, University of Cordoba, Francisco Santisteban Hospital, Campus de Rabanales, 14014 Córdoba, Spain.

The authors:

Juan Morgaz, Sergio Ventura, Pilar Muñoz-Rascón, Rocio Navarrete , José Pérez, María del Mar Granados, José Andrés Fernández-Sarmiento, Juan Manuel Domínguez, Verónica Molina, Rafael J. Gómez-Villamandos and Rafael Zafra.

Intestinal ischemia-reperfusion (IR) is an important clinical occurrence seen in common diseases, such as gastric dilatation-volvulus in dogs or colic in horses. Limited data is available on the use of methylene blue in veterinary medicine for intestinal ischemia-reperfusion. The present study aimed to compare the hemodynamic, histopathological, and immunohistochemical effects of two doses of methylene blue in two rabbit model groups In one group, 5 mg/kg IV was administered, and in another, 20 mg/kg IV was administered following a constant rate infusion (CRI) of 2 mg/kg/h that lasted 6 h. All the groups, including a control group had intestinal ischemia-reperfusion. Immunohistochemical analysis was performed using caspase-3. During ischemia, hemodynamic depression with reduced perfusion and elevated lactate were observed. During reperfusion, methylene blue (MB) infusion generated an increase in cardiac output due to a positive chronotropic effect, an elevation of preload, and an intense positive inotropic effect.