Methylene blue attenuates renal ischemia-reperfusion injury by negative regulation of NLRP3 signaling pathway

Research performed at:

Department of Nephrology, Xiangyang Central Hospital, Affliated Hospital of Hubei College of Arts and Science, Xiangyang, China.

The authors:

j.-j. Liu, l. Lu, f.-q. Hu, h. Yuan, q. Xu, y.-f. Qin, j.-h. Gong.

Objective: To investigate the effect of methylene blue (MB) on renal ischemia-reperfusion (IR) injury in mice and its possible relevant mechanisms.

A total of 30 male C57/BL6 mice aged 4 months old were randomly divided into the following three groups:

  • Sham group (n=10), IR group (n=10).
  • MB group (n=10).

Mice in MB group were treated with gavage continuously using methylene blue solution (dosage: 25 mg·kg -1·d -1) until they were 7 months old.

It was found in this experimental study that Scr and BUN levels in MB group were significantly lower than those in IR group, and the differences were statistically significant (p<0.05)/

Conclusions: We showed that methylene blue can alleviate the apoptosis and inflammatory response induced by renal IR injury in mice, and its relevant mechanism may be related to the fact that methylene blue can negatively regulate NLRP3 signaling pathway.