Research performed at:
Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil.
The authors:
Roberto Ferreira Meirelles Jr, Reginaldo Ceneviva, Fernanda Viaro, Caroline Floreoto Baldo, Paulo Roberto Barbosa Evora.
Study hemodynamic pattern and lipoperoxidation during methylene blue (MB) treatment on taurocholate-enterokinase induced acute pancreatitis (AP).
Thirty pigs were equally divided in control group:
- MB group.
- AP group.
- MB previous AP group.
- MB after 90 min of induced AP group.
MB was given iv in a bolus dose (2mg.kg-1) followed by maintenance dose (2 mg.kg-1.h-1). Hemodynamic parameters were recorded continuously during 180 min by Swan-Ganz catheter. Blood samples were taken every 60 min to determine arterial and venous nitrate, malondialdehyde (MDA) and amylase. Pancreatic tissue was removed for histopathologic study.
In this taurocholate-induced AP model MB treatment delayed hemodynamic shock and decreases serum nitrate levels but increases serum MDA levels. No volemic replacement was done and it may have been a mitigated factor to a poor tissue perfusion and impairment microcirculation. Further investigations are needed to elucidate MB treatment role during AP treatment.