Possibilities of methylene blue in photodynamic inactivation of bacteria in vitro experiment

Research performed at:

1. Tashkent Pediatric Medical Institute.

2. Tashkent State Dental Institute.

The authors:

Sabirmatov A.A, Tursumetov A. A., Mukhamedov I.M., Jafarov Kh.M., Iskhakov Sh. Sh.

The paper presents the results of bench microbiological studies on the study of the antibacterial properties of red spectrum LED radiation with a wavelength of 630 ± 20 nm, the photosensitizer methylene blue (MB) in various concentrations.

The objects of exposure were bacteria sown from hernial water in strangulated hernia and abdominal exudate in peritonitis (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli). It was found that LED radiation and MB, separately, do not possess bactericidal properties.

Photodynamic effect (PDT) (LED radiation in combination with 0.05% MB solution) has a pronounced bactericidal effect. The obtained results are discussed from the point of view of the possibility of using PDT for the prevention of postoperative proinflammatory complications.